Senior Research Assistant
Company: Case Western Reserve University
Location: Lorain
Posted on: January 26, 2023
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Job Description:
Salary Grade
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Salary Grade 12 and staff working 40 hours per week
To view salary ranges or Salary Administration Attachments, please
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Job Description
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POSITION OBJECTIVE
Working under limited supervision, the Research Assistant 3 will
coordinate research work that focuses on identifying the cellular
and molecular mechanisms underlying aggressive forms of breast
cancer(s). The research assistant will maintain cell cultures,
assist with the ongoing animal experiments, and carry out
experiments using Molecular Biology techniques including cloning,
and CRISPR/Cas9. The research assistant will perform data analysis
and may co-author on laboratory publications. This position will
work with animals as well as mouse and human tissue.
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ESSENTIAL FUNCTIONS
Coordinate laboratory/ research activities. Carry out eukaryotic
cell culture and biological experiments independently but with
input of other research scientists. The experiments will be in the
field of cell and radiobiology, such as measuring cell
proliferation rates (cell counting or live cell density
monitoring), cell death (apoptosis by flow cytometry, nuclear
condensation by microscopy, clonogenic survival, etc.), or
immunofluorescence. Maintain cell cultures, including both standard
cell propagation and expansion, as well as advanced techniques
including plasmid or siRNA transfection, lentivirus infection,
invasion/migration assays, and clonogenic survival assays. Carry
out experiments as directed utilizing Molecular Biology techniques
including cloning, CRISPR/Cas9 and insertional mutagenesis. Conduct
in vivo and in vitro radiation experiments using an orthovoltage
radiation machine. (24%) Carry out complex research assignments of
a non-routine nature - Perform in vivo murine techniques. Specific
experimental studies may require mammary fat pad injections and
xenograft studies with drug administration to animals. Perform in
vitro techniques including flow cytometry, PCR, cell culture,
western blot, cell growth, etc. (20%) Work closely with the
principal investigator to carry out complex research including
administration of medications, chemical reagents, or biologic
agents, handling cells, tissues, and animals per IACUC protocols
and laboratory methodology. Perform cell culture, tissues harvest
and acquisition, DNA/RNA/protein extraction. (20%) Devise new
protocols and techniques for research projects involving a high
degree of skill and training; these include characterizing the
therapeutic benefit of specific inhibitors on radio sensitization.
(10%) Evaluate adequacy of techniques and provide feedback on
experimental design; optimize and troubleshoot procedures to comply
with expected outcomes. (7%) Train new staff regarding standard
laboratory policies as well as basic radiobiology research, and
provide support to other research assistants. Keep a clear and
complete laboratory notebook. Responsible for cell culture
maintenance tasks including water bath and cell culture incubator
maintenance, lab ordering, supply re-stocking, and lab tidiness.
May monitor the laboratory budget. (10%) May co-author research
projects. Participate in manuscript and grant writing, co-author
research projects and provide data to the principal investigator
for sponsor progress reports, manuscripts, grant and pilot
applications. (6%)
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NONESSENTIAL FUNCTIONS
Attend departmental functions and perform other duties as assigned.
(3%)
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CONTACTS
Department: Daily contact with the lab principal investigator
regarding research.
University: Occasional contact with other departments to share
information on collaborative projects.
External: Limited contact with vendors to exchange information and
order supplies.
Students: Contact with student employees to provide training or
direct research.
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SUPERVISORY RESPONSIBILITY
This position has no direct supervision of staff employees. May
supervise students and technicians.
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QUALIFICATIONS
Experience: 3 to 5 years of related experience required. Working
knowledge of biomedical research and cell culture or similar
biological experiments with previous lab experience preferred.
Education: Bachelor's degree in science required.
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REQUIRED SKILLS
Knowledge of commonly-used concepts, practices, and procedures used
in molecular and radiobiology. Knowledge of Molecular Biology
techniques including cloning, CRISPR/Cas9 and insertional
mutagenesis. Must have the ability to maintain meticulous,
complete, and easily retrievable laboratory data. Must demonstrate
compliance with Case Western Reserve University animal research and
care (ARC) policies and procedures and compliance to regulations of
the Animal Welfare Act, Public Health Service Policy, AAALAC
guidelines and other applicable regulatory guidelines. Must
demonstrate compassion for animals within Case Western Reserve
University facilities and dedication to the Animal Resource
Center's mission. Must handle animals with care and respect at all
times. Must be able and willing to learn new techniques,
procedures, processes, and computer gear to protect the health of
the animals. Previous experience working with animals preferred.
Ability to rely on instructions and pre-established guidelines to
perform the functions of the job. Ability to operate laboratory
equipment. Ability to work effectively independently and
collaboratively within a team. Proficiency in Microsoft Office and
GraphPad Prism is preferred. Experience using Adobe Photoshop,
Illustrator, and FlowJo is a plus. Must demonstrate willingness to
learn new techniques, procedures, processes and computer programs
as needed. Ability to interact effectively with colleagues,
supervisors and students face to face. Ability to meet consistent
attendance.
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WORKING CONDITIONS
General laboratory environment. May have exposure to hazards
including routine laboratory chemicals and solvents. Personal
protective equipment, including lab coat, gloves and goggles, will
be provided to limit exposure to hazards.
Case Western Reserve University's animal facilities are accredited
by the Association for the Assessment and Accreditation of
Laboratory Animal Care (AAALAC) and is managed according to the
"Guide for the Care and Use of Laboratory Animals" appropriate
Federal Animal Welfare Regulations, and the Public Health Service
"Policy on the Humane Care and Use of Laboratory Animals." This
position, and all animal research personnel, are subject to
internal compliance to Animal Resource Center Standard Operating
Procedures and to compliance regulations of the Animal Welfare Act,
Public Health Service Policy, AAALAC guidelines, the State of Ohio
Veterinary Practice Act, Federal Drug Enforcement Administration
regulatory guidelines, US Food and Drug Administration Center for
Veterinary Medicine regulations and other applicable regulatory
guidelines.
Diversity Statement
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In employment, as in education, Case Western Reserve University is
committed to Equal Opportunity and Diversity. - Women, veterans,
members of underrepresented minority groups, and individuals with
disabilities are encouraged to apply.
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Reasonable Accommodations
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Case Western Reserve University provides reasonable accommodations
to applicants with disabilities. - Applicants requiring a
reasonable accommodation for any part of the application and hiring
process should contact the Office of Equity -at 216-368-3066 -to
request a reasonable accommodation. - Determinations as to granting
reasonable accommodations for any applicant will be made on a
case-by-case basis.
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COVID Vaccination
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Case Western Reserve University has implemented a COVID Vaccination
policy requiring evidence of COVID-19 vaccination for all students,
faculty and staff with an on-campus presence. Religious and medical
exemptions may be provided in accordance with applicable federal
and state laws. For more information go to:
https://case.edu/equity/covid-19-accommodations. Applicants may
contact the Office of Equity at 216-368-3066 /equity@case.edu for
information regarding religious or medical exemptions as an
accommodation.
Keywords: Case Western Reserve University, Lorain , Senior Research Assistant, Other , Lorain, Ohio
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